Biochemistry was introduced in University of Lucknow in 1955 as one of the subjects for specialization in M.Sc. (Chemistry). The Department made a modest beginning by occupying a few rooms in the building of the Chemistry Department in the erstwhile combined Departments of Chemistry and Biochemistry of the University. A separate Department of Biochemistry came into existence in August 1963 and it was shifted to its present premises in 1966. Generous grants from the University Grants Commission and the Rockefeller Foundation enabled the construction of the building for the Department and the acquisition of essential equipment.
The teaching of Biochemistry started in July 1955 with Dr. Soma Kumar, then lecturer in Organic Chemistry. Subsequently, with the appointment of Dr. P. S. Krishnan as Reader in Biochemistry in 1956, teaching in Biochemistry came to full swing. Dr. G.G. Sanwal joined the Department in 1957, followed by Dr. K.K. Tewari in 1959. Dr. P.S. Krishnan became Professor in Biochemistry in the Chemistry Department in 1958. At that time the number of students admitted to M.Sc. was only ten and the staff consisted of only one Professor and two Lecturers. Prof. P.S. Krishnan was appointed Head of the Department of Biochemistry on August 17, 1963. He has been the architect for the creation and establishment of the Department of Biochemistry. His profound scholarship, planning and vision have been mainly responsible for widening the horizon of activities in the Department and its subsequent expansion. After the superannuation of Prof. P.S. Krishnan, Prof. G.G. Sanwal took over as the Head of the Department and continued till 1996. In addition to SAP, he was also able to obtain several grants from agencies such as UNESCO and COSIST. Prof. K.C.S. Sanger headed the Department from 1996 to 1997. Prof. P.C. Misra took over as the Head of the Department in December 1997 and remained in that position till November 2006. This period was marked by getting financial assistance from Department of Science and Technology (DST) under its FIST Programme. A separate computer laboratory with internet facility was also established in 2004 under this support.
Prof. U. N. Dwivedi took over as the Head of the Department of Biochemistry in November 2006 and continued till November 2009. Prof. Dwivedi initiated research and teaching in the area of Biotechnology since 1993 with major fundings received from Department of Biotechnology (DBT) and from CSIR under NMITLI networking program. The M.Sc. Biotechnology teaching program, with Prof. U.N. Dwivedi as the Co-ordinator, started from academic session 2002-2003. This is a four semester program financially supported by Department of Biotechnology (DBT), Govt. of India and ten students are admitted every year through the common National Entrance Test conducted by JNU, New Delhi. The Department also introduced Biotechnology as one of the subjects at the undergraduate level from the academic session 2007-2008. This undergraduate program continued till 2012. The period was also marked by getting grants from DBT for development of a Bioinformatics Facility in the Department. The Department has also received Centre of Excellence Grant in the area of Bioinformatics from U.P. State Govt. in the year 2009.
After Prof. U.N. Dwivedi, Prof. R.K. Mishra headed the Department from November 2009 to January 2010 followed by Prof. V.K. Tandon, the then Dean faculty of science for another two months. In March 2010, Prof. S.K. Agarwal has become the Head of the Department followed by Prof. R.K. Mishra from March 2013 to March 2016. Presently Prof. Deepak Chandra is heading the Department since 30th March 2016 The Department has also received Centre of Excellence Grant in the field of Proteomics, Protein Engineering and Enzymology from U.P. State Govt. under his leadership. The Department has been receiving funds and extramural research grants from various funding agencies such as UGC, CSIR, CSIR-NMITLI, DST, DBT, ICMR, UPCST, AICTE, etc. The Department has fully equipped laboratories which include labs for plant tissue culture, animal cell culture and also labs for proteomic and genomic studies. The state of the art equipment such as real time PCR (RT-PCR), thermal cycler, ballistic gene gun, HPLC, multiplex gel documentation system, ultracentrifuges, UV cross linker, deep freezers, fermenter, 2D gel electrophoresis system etc. are available in the Department. The students and faculty members have been publishing research papers in national and international journals of high repute, in various thrust areas of research such as plant biochemistry and biotechnology, enzymology, mechanistic aspects of diabetic complications, membrane biology, photobiology, cancer molecular biology etc. Till date, more than 200 students have been awarded Ph.D. degree from this Department.